What is the Sleeping Beauty (SB) transposon system?
Q: What is the Sleeping Beauty (SB) transposon system?
A: The Sleeping Beauty (SB) transposon system is a non-viral vector for gene therapy that enables high-level stable gene transfer and sustained transgene expression in multiple primary human somatic cell types.
Q: Why is the SB system an attractive gene transfer strategy for clinical use?
A: The SB system is attractive because it enables stable gene transfer and long-term expression of the gene of interest in human cells.
Q: What are the two components of the SB transposon-based transfection system?
A: The two components of the SB transposon-based transfection system are a transposase and a transposon containing inverted repeat/direct repeat (IR/DR) sequences.
Q: How does the SB transposon system ensure precise integration into a TA dinucleotide?
A: The SB transposon system ensures precise integration into a TA dinucleotide because the transposon is designed with an expression cassette of interest flanked by IR/DRs, and the SB transposase mediates stable integration.
Q: What is the role of the transposon in the SB system?
A: The transposon in the SB system contains an expression cassette of interest flanked by IR/DRs and mediates stable integration and reliable long-term expression of the gene of interest.
Q: Can the SB system efficiently mediate gene transfer in human embryonic stem (ES) cells?
A: Yes, recent research has found that the SB system efficiently mediates gene transfer and stable gene expression in human embryonic stem (ES) cells.
Q: What is the source of information for the text about the SB system?
A: The source of information for the text about the SB system includes various research papers published on PubMed and CSH Protocols.
