Lafayette County is a county in the state of Florida. As of 2000, 7,022 people lived there. It is the second least populated county in the state according to the 2000 census, because it has one more resident than Liberty County, Florida. The U.S. Census Bureau 2005 estimate for the county is 7,773. Its county seat is Mayo, Florida. Lafayette County is a prohibition county, meaning alcohol is illegal there.