Colbert County is a county of the U.S. state of Alabama. Its name is in honor of brothers George and Levi Colbert, Chickasaw Indian chiefs. George Colbert operated a ferry across the Tennessee River in 1790 near present-day Cherokee.

The county seat of Colbert County is Tuscumbia. It is part of the Florence - Muscle Shoals Metropolitan Statistical Area known as "The Shoals". As of 2010, 54,428 people lived there.