The Cincinnati Zoo and Botanical Garden is a zoo in the American city of Cincinnati, Ohio. It claims to be the second oldest zoo in the United States because it opened 14 months after the Philadelphia Zoo.

The zoo has over 65.4 acres (26.5 ha) of land and about 2,800 animals. It has over 30 indoor and outdoor attractions. The Cincinnati Zoo also breeds rare animals. For example, it was the first zoo to breed the California sea lion and one of the first to breed the American bison both in 1878. It is the only place in the Western Hemisphere where one, two, and three Sumatran Rhinoceroses have been born.