Q: What is Zinc finger nucleases or ZFN?
A: Zinc finger nucleases or ZFN is a tool used to target genes and change DNA.
Q: What are the three methods of changing the genome with engineered nucleases?
A: Zinc finger nucleases or ZFN, Transcription Activator-Like Effector Nucleases (TALEN), and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are the three methods of changing the genome with engineered nucleases.
Q: What are zinc fingers?
A: Zinc fingers are man-made molecules made of a protein and zinc which bind to a specific DNA.
Q: What are Zinc-finger nucleases (ZFNs)?
A: Zinc-finger nucleases (ZFNs) are enzymes that result from fusing a zinc finger to a DNA cleavage enzyme called a Fokl.
Q: What happens when the ZFN binds onto a specific DNA sequence?
A: When the ZFN binds onto a specific DNA sequence, it snips it in two places.
Q: What do regular cell enzymes do after the DNA is snipped out?
A: Regular cell enzymes stick the ends together, minus the bit snipped out (DNA repair).
Q: What are the two types of DNA changes that Zinc finger nucleases or ZFN can make?
A: Zinc finger nucleases or ZFN can make two types of DNA changes: mutations such as deletions and insertions.