What is transfection?

Q: What is transfection?


A: Transfection is the process of deliberately introducing DNA or RNA into cells.

Q: How is the term "transfection" formed?


A: The term "transfection" is formed from the words "transformation" and "infection."

Q: What are the different types of transfection?


A: The different types of transfection include: transformation of bacterial cells with viral nucleic acids, transformation of animal cells in tissue culture with purified DNA, transformation of cells or embryos with single or double-stranded RNA, and gene therapy using a modified virus as a vector.

Q: What happens when DNA is added to the cells' genome during transfection?


A: When DNA is added to the cells' genome during transfection, it can cause the building of particular proteins or the silencing of particular genes.

Q: Can changes produced by transfection with RNA molecules be permanently transmitted down a line of cells?


A: No, changes produced by transfection with RNA molecules cannot be permanently transmitted down a line of cells.

Q: What can transfection result in?


A: Transfection can result in unexpected morphologies and abnormalities in target cells.

Q: What is the purpose of gene therapy using a modified virus as a vector?


A: The purpose of gene therapy using a modified virus as a vector is to introduce new, functional genes into the genome of an individual with a genetic disease, in the hopes of correcting or treating the disease.

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